Modeling and Optimization of 1-Kestose Production by Schedonorus arundinaceus 1-SST
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Tipo do ITEM
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Artigo Ciêntifico
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Título do Artigo
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Modeling and Optimization of 1-Kestose Production by Schedonorus arundinaceus 1-SST
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Descrição
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In current commercial mixtures of inulin-type fructooligosaccharides (FOS) synthesized from sucrose by fungal fructosyltransferases, 1-kestose has superior bifidus-stimulating effect than nystose and frutosyl-nystose. In this study,
a recombinant sucrose:sucrose 1-fructosyltransferase (1-SST, EC 2.4.1.99) from the plant Schedonorus arundinaceus (Sa)
was the enzyme of choice to maximize the batch production of 1-kestose in a jacketed agitated vessel. Mathematical
models were developed to predict the optimal conditions for the sucrose conversion reaction and the subsequent
enzyme inactivation to prevent 1-kestose hydrolysis. After programed heat inactivation of Sa1-SSTrec in optimized
batch experiments with different enzyme and sucrose concentrations, 1-kestose represented more than 90% of total
FOS content (53%-58%, w/w) in the reaction mixture. The mathematical models described herein are suitable tools
for the cost-effective production of 1-kestose in scaled batch reactions.
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Abstract
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In current commercial mixtures of inulin-type fructooligosaccharides (FOS) synthesized from sucrose by fungal fructosyltransferases, 1-kestose has superior bifidus-stimulating effect than nystose and frutosyl-nystose. In this study,
a recombinant sucrose:sucrose 1-fructosyltransferase (1-SST, EC 2.4.1.99) from the plant Schedonorus arundinaceus (Sa)
was the enzyme of choice to maximize the batch production of 1-kestose in a jacketed agitated vessel. Mathematical
models were developed to predict the optimal conditions for the sucrose conversion reaction and the subsequent
enzyme inactivation to prevent 1-kestose hydrolysis. After programed heat inactivation of Sa1-SSTrec in optimized
batch experiments with different enzyme and sucrose concentrations, 1-kestose represented more than 90% of total
FOS content (53%-58%, w/w) in the reaction mixture. The mathematical models described herein are suitable tools
for the cost-effective production of 1-kestose in scaled batch reactions.
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Língua do arquivo
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inglês
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Data da Publicação
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Ano 2022
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Palavra-chave
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Thermal conductivity
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Kinetic modeling
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Fructooligosaccharides
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Autores
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Duniesky Martínez
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Roberto J. Cabrera
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Iván Rodríguez
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Carmen Menéndez
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Alina Sobrino
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Lázaro Hernández
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Enrique R. Pérez
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Local
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ITEGAM - CUBA, 2022
